LIMS1 Antikörper (AA 11-172)

Produktnummer ABIN7870068

Der Kaninchen Polyklonal anti-LIMS1 Antikörper wird verwendet zum Nachweis von LIMS1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für LIMS1 Antikörper (AA 11-172) (ABIN7870068)

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIMS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-LIMS1 Antikörper

Bindungsspezifität: AA 11-172

Verwendungszweck: LIMS1 Antibody / LIM and senescent cell antigen-like-containing domain protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LIMS1 recombinant protein (Position: E11-A172) was used as the immunogen for the LIMS1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LIMS1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LIMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LIMS1

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Andere Bezeichnung: LIMS1

Hintergrund: LIMS1 antibody detects LIM and senescent cell antigen-like-containing domain protein 1, encoded by the LIMS1 gene on chromosome 2q12.3. LIMS1 antibody is widely applied in studies of integrin signaling, cell adhesion, and cancer biology. LIMS1, also known as PINCH1, is a LIM domain-containing adaptor protein that localizes to focal adhesions, where it connects integrins with the actin cytoskeleton and signaling pathways. It plays a central role in cell-matrix interactions and regulation of survival, proliferation, and migration.

Structurally, LIMS1 is a ~37 kDa protein composed of five LIM domains, which mediate protein-protein interactions. Through these domains, LIMS1 interacts with integrin-linked kinase (ILK) and parvin to form the IPP complex, a key signaling hub at focal adhesions. This complex links extracellular matrix adhesion with intracellular signaling cascades.

Functionally, LIMS1 stabilizes focal adhesions and promotes integrin-mediated signaling. It regulates pathways including PI3K-Akt, MAPK, and Rho GTPase signaling, influencing survival, motility, and cytoskeletal organization. Knockout models demonstrate that LIMS1 is essential for embryonic development and cell viability. Researchers use LIMS1 antibody to investigate adhesion signaling, integrin function, and tumor progression.

Clinically, LIMS1 is associated with cancer, where its overexpression promotes invasion and metastasis by enhancing integrin signaling. It also influences therapy resistance in multiple tumor types. Genetic studies have linked LIMS1 variants to immune function and kidney transplantation outcomes, suggesting roles beyond adhesion. NSJ Bioreagents supplies LIMS1 antibody for use in adhesion biology, immunology, and oncology.

Experimentally, LIMS1 antibody is applied in western blotting to detect the ~37 kDa protein, in immunofluorescence to visualize focal adhesion localization, and in immunohistochemistry to assess tumor expression. Co-immunoprecipitation with LIMS1 antibody identifies ILK, parvin, and integrin-binding partners.

UniProt: P48059