SSH3 Antikörper (AA 108-620)

Produktnummer ABIN7870034

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SSH3 in WB, ELISA, IHC, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für SSH3 Antikörper (AA 108-620) (ABIN7870034)

Target: SSH3 (Slingshot Homolog 3 (SSH3))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SSH3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-SSH3 Antikörper

Bindungsspezifität: AA 108-620

Verwendungszweck: SSH3 Antibody / Slingshot homolog 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SSH3 recombinant protein (Position: Q108-Q620) was used as the immunogen for the SSH3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SSH3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SSH3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SSH3

Target: SSH3 (Slingshot Homolog 3 (SSH3))

Andere Bezeichnung: SSH3

Hintergrund: SSH3 antibody detects Slingshot homolog 3, a member of the slingshot family of serine/threonine phosphatases that regulate actin filament dynamics through dephosphorylation of cofilin. The UniProt recommended name is Slingshot homolog 3 (SSH3). This phosphatase modulates cytoskeletal reorganization, cell migration, and synaptic remodeling by maintaining the balance between phosphorylated and active cofilin.

Functionally, SSH3 antibody identifies a 575-amino-acid cytoplasmic enzyme that acts downstream of Rho family GTPases. SSH3 dephosphorylates cofilin at serine 3, restoring its actin-severing activity and promoting filament turnover. The protein also interacts with scaffolding components such as 14-3-3 proteins and LIM kinases, coordinating actin remodeling in response to extracellular cues.

The SSH3 gene is located on chromosome 3p21.31 and is widely expressed in brain, muscle, and epithelial tissues. SSH3 contributes to cell shape changes, migration, and polarity through dynamic actin regulation. In neurons, it participates in synaptic plasticity and axon guidance by fine-tuning cytoskeletal rearrangements during development and signaling.

Pathologically, aberrant SSH3 activity disrupts cytoskeletal organization and has been associated with cancer metastasis and neurological disorders. Overactivation enhances cell motility and invasion, whereas loss of function impairs normal morphogenesis. Research with SSH3 antibody supports studies on actin regulation, neuronal development, and signal transduction.

SSH3 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry, enabling precise detection of SSH3 in various cell types. NSJ Bioreagents provides SSH3 antibody reagents validated for actin cytoskeleton and phosphatase signaling research.

Structurally, SSH3 contains an N-terminal catalytic domain related to the dual-specificity phosphatase family and C-terminal regions mediating protein-protein interactions. Its localization and activity are regulated by phosphorylation and 14-3-3 binding. This antibody facilitates analysis of SSH3's role in actin filament turnover and cell motility control.

UniProt: Q8TE77