TTL Antikörper (AA 104-377)

Produktnummer ABIN7869968

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TTL in ELISA und WB. Es zeigt Reaktivität gegenüber Proben von Maus und Ratte.

Kurzübersicht für TTL Antikörper (AA 104-377) (ABIN7869968)

Target: TTL (Tubulin tyrosine Ligase (TTL))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TTL Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-TTL Antikörper

Bindungsspezifität: AA 104-377

Verwendungszweck: TTL Antibody / Tubulin-tyrosine ligase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TTL recombinant protein (Position: N104-L377) was used as the immunogen for the TTL antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TTL antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TTL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TTL

Target: TTL (Tubulin tyrosine Ligase (TTL))

Andere Bezeichnung: TTL

Hintergrund: TTL antibody detects Tubulin-tyrosine ligase, an essential enzyme responsible for post-translational modification of alpha-tubulin through the addition of a C-terminal tyrosine residue. The UniProt recommended name is Tubulin-tyrosine ligase (TTL). This cytosolic enzyme catalyzes the ATP-dependent retyrosination of detyrosinated alpha-tubulin, maintaining the dynamic equilibrium between tyrosinated and detyrosinated microtubules crucial for cytoskeletal organization and intracellular transport.

Functionally, TTL antibody identifies a 377-amino-acid enzyme that activates free tyrosine and transfers it to the exposed C-terminal glutamate of alpha-tubulin. This reversible modification, known as the tubulin tyrosination cycle, is fundamental to microtubule turnover, stability, and interactions with motor proteins such as kinesin and dynein. TTL activity regulates cell polarity, mitotic spindle assembly, and vesicle trafficking by modulating microtubule dynamics.

The TTL gene is located on chromosome 2q13 and is ubiquitously expressed, with high levels in brain, heart, and skeletal muscle. TTL's enzymatic cycle complements that of tubulin carboxypeptidase, ensuring balanced tyrosination of alpha-tubulin. This modification acts as a spatial cue for motor proteins and microtubule-associated proteins that distinguish dynamic from stable microtubules.

Pathologically, loss or suppression of TTL expression has been linked to tumor progression, neurodegeneration, and impaired axonal transport. In cancer, reduced TTL correlates with increased detyrosinated tubulin, enhancing cell migration and invasion. In neurons, TTL deficiency disturbs axonal guidance and synaptic function. Research using TTL antibody helps elucidate these processes by revealing spatial and temporal patterns of tubulin modification.

TTL antibody is suitable for western blotting, immunocytochemistry, and immunofluorescence to detect tyrosinated tubulin and monitor microtubule dynamics. NSJ Bioreagents provides validated TTL antibody reagents optimized for studies in cytoskeletal regulation, neurobiology, and cancer cell biology.

Structurally, TTL contains an ATP-grasp catalytic fold that binds tyrosine and alpha-tubulin. The enzyme's activity depends on its recognition of the unmodified C-terminus of alpha-tubulin and coordination of divalent cations for catalysis. This antibody enables researchers to investigate tubulin modification, microtubule behavior, and cellular architecture regulation.

UniProt: Q8NG68

Pathways: Regulation of Cell Size