AQP2 Antikörper (AA 102-271)

Produktnummer ABIN7869912

Der Kaninchen Polyklonal anti-AQP2 Antikörper wird verwendet zum Nachweis von AQP2 in Proben von Ratte und Maus. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für AQP2 Antikörper (AA 102-271) (ABIN7869912)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: AA 102-271

Verwendungszweck: Aqp2 Antibody / Aquaporin 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271) was used as the immunogen for the Aqp2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Aqp2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Aqp2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aqp2

Hintergrund: The AQP2 antibody targets Aquaporin-2, a water channel protein encoded by the AQP2 gene. Aquaporin-2 is an essential component of the kidney's water reabsorption system, located in the apical membrane of collecting duct principal cells. It mediates vasopressin-regulated water transport, thereby controlling urine concentration and maintaining body fluid balance. The AQP2 antibody enables researchers to study renal physiology, osmoregulation, and disorders of water homeostasis such as diabetes insipidus.

Aquaporin-2 belongs to the aquaporin family of integral membrane proteins that selectively transport water across biological membranes. Under the influence of the antidiuretic hormone vasopressin, AQP2-containing vesicles are trafficked to and fused with the apical plasma membrane, increasing water permeability. The AQP2 antibody provides a reliable means to visualize this regulated translocation process, making it a key tool for examining vasopressin signaling and collecting duct responsiveness.

Mutations in the AQP2 gene lead to nephrogenic diabetes insipidus (NDI), characterized by excessive urine production and an inability to concentrate urine despite normal vasopressin levels. The AQP2 antibody supports research into this condition by enabling detection of defective or mislocalized Aquaporin-2 in renal tissue. Experimental models show that AQP2 deficiency disrupts water retention and contributes to dehydration, underscoring its clinical relevance in renal water management.

Beyond kidney physiology, Aquaporin-2 has been detected in reproductive tissues and the central nervous system, suggesting broader roles in fluid transport and homeostasis. The AQP2 antibody supports such investigations, allowing comparative expression analysis across tissues and experimental conditions. Regulation of AQP2 expression and trafficking involves multiple pathways including cyclic AMP, protein kinase A, and ubiquitination-dependent endocytosis.

The AQP2 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, producing distinct apical membrane staining in kidney tissue. NSJ Bioreagents provides this antibody with verified specificity and reproducibility for renal physiology, endocrinology, and molecular signaling research. By enabling precise detection of Aquaporin-2, the AQP2 antibody advances understanding of water transport regulation and the molecular mechanisms underlying disorders of water balance.

UniProt: P56402

Pathways: Response to Water Deprivation