TIMM8B Antikörper (AA 1-83)

Produktnummer ABIN7869574

Der Kaninchen Polyklonal anti-TIMM8B Antikörper (ABIN7869574) detektiert spezifisch TIMM8B in ELISA, WB und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für TIMM8B Antikörper (AA 1-83) (ABIN7869574)

Target: TIMM8B (Translocase of Inner Mitochondrial Membrane 8 Homolog B (TIMM8B))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIMM8B Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-TIMM8B Antikörper

Bindungsspezifität: AA 1-83

Verwendungszweck: TIMM8B Antibody / Tim8 B

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TIMM8B recombinant protein (Position: M1-Q83) was used as the immunogen for the TIMM8B antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TIMM8B antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TIMM8B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TIMM8B

Target: TIMM8B (Translocase of Inner Mitochondrial Membrane 8 Homolog B (TIMM8B))

Andere Bezeichnung: TIMM8B

Hintergrund: TIMM8B antibody detects Mitochondrial import inner membrane translocase subunit Tim8B, a small chaperone-like protein that assists in importing and assembling hydrophobic carrier proteins into the mitochondrial inner membrane. Encoded by the TIMM8B gene on chromosome 11q14.1, this protein belongs to the mitochondrial intermembrane space (IMS) chaperone complex family, which also includes Tim8A, Tim9, and Tim10. TIMM8B forms part of the TIM8-TIM13 complex that stabilizes and delivers precursor proteins across the IMS to the TIM22 translocase complex, ensuring proper mitochondrial biogenesis and energy metabolism.

Structurally, TIMM8B contains conserved twin CX3C motifs that coordinate zinc ions, stabilizing its folding and interaction with partner subunits. Within the IMS, TIMM8B associates with TIMM13 to form a 70 kilodalton heterohexameric complex. This assembly binds and shuttles hydrophobic transmembrane segments of carrier proteins, preventing their aggregation during translocation. Loss or dysfunction of TIMM8B disrupts mitochondrial protein import, leading to defects in oxidative phosphorylation and metabolic balance. While TIMM8A mutations cause Mohr-Tranebjaerg syndrome, TIMM8B plays a complementary and partially compensatory role in mitochondrial maintenance.

The TIMM8B antibody is widely used in cell biology, mitochondrial, and neurodegeneration research to study protein import mechanisms and mitochondrial function. Western blot analysis identifies a 10 kilodalton band corresponding to TIMM8B, while immunofluorescence reveals punctate mitochondrial localization that colocalizes with markers such as TOM20 and COX IV. This antibody enables precise examination of mitochondrial translocase assembly and protein trafficking. Because mitochondrial dysfunction contributes to neurodegenerative disorders, TIMM8B detection provides valuable insights into pathophysiology and energy metabolism.

Beyond its role in import, TIMM8B contributes to mitochondrial quality control and stress response by maintaining the stability of the carrier import pathway. It supports the biogenesis of metabolite transporters such as ANT, MPC, and phosphate carriers. The TIMM8B antibody supports detailed analysis of these pathways and is validated by NSJ Bioreagents for its applications, ensuring reproducible results in mitochondrial and cellular metabolism studies.

UniProt: Q9Y5J9

Pathways: Sensory Perception of Sound, SARS-CoV-2 Protein Interaktom