NUP93 Antikörper (AA 1-677)

Produktnummer ABIN7869524

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NUP93 in WB, ELISA, IHC, IP, FACS, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NUP93 Antikörper (AA 1-677) (ABIN7869524)

Target: NUP93 (Nucleoporin 93kDa (NUP93))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUP93 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-NUP93 Antikörper

Bindungsspezifität: AA 1-677

Verwendungszweck: NUP93 Antibody / Nuclear pore complex protein Nup93

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NUP93 recombinant protein (Position: M1-Q677) was used as the immunogen for the NUP93 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUP93 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUP93 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUP93

Target: NUP93 (Nucleoporin 93kDa (NUP93))

Andere Bezeichnung: NUP93

Hintergrund: NUP93 antibody detects Nuclear pore complex protein Nup93, encoded by the NUP93 gene on chromosome 16p13.11. NUP93 antibody is widely used to study nucleocytoplasmic transport, nuclear pore structure, and gene regulation. NUP93 is a core scaffold nucleoporin essential for assembly and function of the nuclear pore complex (NPC). It contributes to selective transport of macromolecules between the nucleus and cytoplasm, maintaining nuclear integrity and regulating gene expression. NUP93 expression is ubiquitous, consistent with its fundamental role in cell viability.

Structurally, NUP93 is a ~93 kDa protein with coiled-coil domains that mediate interactions with other nucleoporins. It forms part of the inner ring of the NPC, interacting with NUP205, NUP188, and NUP62. These associations stabilize the pore scaffold and provide docking sites for transport factors. Mutations in NUP93 disrupt NPC assembly, impairing nuclear transport and transcriptional regulation.

Functionally, NUP93 regulates nuclear transport by forming the selective barrier that allows import of nuclear proteins and export of RNA. It also participates in mitosis, where disassembly and reassembly of nuclear pores are tightly controlled. NUP93 influences chromatin organization and transcription by anchoring regulatory proteins at the nuclear periphery. Knockdown of NUP93 leads to nuclear transport defects, growth arrest, and apoptosis. Researchers use NUP93 antibody to explore nuclear transport, chromatin regulation, and nuclear architecture.

Clinically, NUP93 mutations are linked to steroid-resistant nephrotic syndrome, a kidney disorder characterized by proteinuria and renal failure. Deficiency disrupts nuclear pore assembly in podocytes, impairing kidney function. NUP93 has also been associated with developmental abnormalities and cancer, where altered nuclear transport contributes to oncogenesis. NSJ Bioreagents provides NUP93 antibody as a reagent for nuclear transport and disease-related studies.

Experimentally, NUP93 antibody is applied in western blotting to detect the ~93 kDa protein, in immunofluorescence microscopy to visualize nuclear pores, and in immunohistochemistry to assess tissue distribution. Immunoprecipitation with NUP93 antibody isolates nuclear pore complexes, supporting biochemical studies of transport machinery.

UniProt: Q8N1F7