FBXW8 Antikörper (AA 1-598)

Produktnummer ABIN7869489

Der Kaninchen Polyklonal anti-FBXW8 Antikörper wird verwendet zum Nachweis von FBXW8 in Proben von Human und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für FBXW8 Antikörper (AA 1-598) (ABIN7869489)

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FBXW8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-FBXW8 Antikörper

Bindungsspezifität: AA 1-598

Verwendungszweck: FBXW8 Antibody / F-box/WD repeat-containing protein 8

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human FBXW8 recombinant protein (Position: M1-V598) was used as the immunogen for the FBXW8 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the FBXW8 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the FBXW8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu FBXW8

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Andere Bezeichnung: FBXW8

Hintergrund: FBXW8 antibody detects F-box/WD repeat-containing protein 8, a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. The UniProt recommended name is F-box/WD repeat-containing protein 8 (FBXW8). This protein mediates selective ubiquitination and degradation of regulatory proteins that control cell cycle progression, growth, and signal transduction.

Functionally, FBXW8 antibody identifies a 616-amino-acid protein that contains an N-terminal F-box motif and multiple C-terminal WD repeats. The F-box domain binds to the SKP1 adaptor protein, anchoring FBXW8 within the SCF complex, while the WD repeats mediate substrate recognition. FBXW8 acts as a specificity factor that determines which proteins are targeted for ubiquitin-dependent degradation. Key substrates include IRS1, TSC2, and cyclin D1, linking FBXW8 to insulin signaling and cell cycle regulation.

The FBXW8 gene is located on chromosome 12q24.31 and encodes a cytoplasmic and nuclear protein expressed in most tissues, particularly in skeletal muscle and liver. FBXW8 associates with the cullin family protein CUL7 to form the CUL7-FBXW8 E3 ligase complex, distinct from classical SCF ligases. This complex regulates growth factor signaling and metabolic homeostasis by mediating the ubiquitination of signaling intermediates involved in mTOR and PI3K pathways.

In physiology, FBXW8 controls insulin receptor signaling by targeting IRS1 for degradation, preventing excessive insulin pathway activation. It also influences neuronal development and differentiation through ubiquitin-mediated turnover of growth-regulating proteins. Disruption of FBXW8 function leads to aberrant signaling, impaired growth, and metabolic imbalance. In cancer, altered FBXW8 expression contributes to tumor cell proliferation and metastasis by deregulating cyclin and kinase levels.

FBXW8 antibody is widely used in cell cycle, signal transduction, and ubiquitin pathway research. It is suitable for immunoblotting, immunoprecipitation, and proteomic studies to identify protein interactions and degradation targets. This antibody provides a valuable tool for investigating the role of F-box proteins in proteostasis and oncogenic signaling. In metabolic studies, FBXW8 detection helps assess the regulation of insulin signaling components.

Structurally, FBXW8's WD-repeat domain forms a beta-propeller structure that enables substrate recognition, while its F-box motif mediates SCF complex assembly. FBXW8 activity is modulated by phosphorylation, ubiquitination, and association with CUL7. NSJ Bioreagents provides FBXW8 antibody reagents validated for use in E3 ligase biology, proteasomal degradation, and metabolic signaling research.

UniProt: Q8N3Y1