RNF220 Antikörper (AA 1-566)

Produktnummer ABIN7869477

Der Kaninchen Polyklonal anti-RNF220 Antikörper wird verwendet zum Nachweis von RNF220 in Proben von Human, Maus und Ratte. Er wurde validiert für ELISA, WB, IF, IHC, FACS und ICC.

Kurzübersicht für RNF220 Antikörper (AA 1-566) (ABIN7869477)

Target: RNF220 (Ring Finger Protein 220 (RNF220))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF220 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-RNF220 Antikörper

Bindungsspezifität: AA 1-566

Verwendungszweck: RNF220 Antibody / Ring finger protein 220

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human RNF220 recombinant protein (Position: M1-L566) was used as the immunogen for the RNF220 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RNF220 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the RNF220 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu RNF220

Target: RNF220 (Ring Finger Protein 220 (RNF220))

Andere Bezeichnung: RNF220

Hintergrund: RNF220 Antibody is used to investigate Ring finger protein 220, a RING-type E3 ubiquitin ligase that modulates the stability and activity of nuclear proteins involved in transcription and development. Ring finger protein 220 contains a C-terminal RING domain that recruits E2 conjugating enzymes and catalyzes ubiquitin transfer to substrates, altering their turnover or interactions. Through these ubiquitin signals, Ring finger protein 220 can fine-tune pathways governing neuronal differentiation, cell cycle progression, and stress responses. Studies in mammalian models implicate Ring finger protein 220 in shaping transcriptional networks during neural development and in regulating signaling modules linked to chromatin state and gene expression.

At the cellular level, Ring finger protein 220 localizes to the nucleus and nuclear lamina, where it participates in multiprotein complexes that coordinate transcriptional repression and activation. Depending on context, Ring finger protein 220 may promote degradation of certain repressors or stabilize selected activators, thereby steering lineage programs and adaptive responses. In addition, Ring finger protein 220 has been connected to canonical Wnt readouts in some systems, consistent with its broader role in influencing transcription factor availability. Because E3 ligases are nodal points in proteostasis and signaling, their measurement can reveal how protein quality control intersects with cell identity and disease.

Researchers apply RNF220 Antibody in immunoblotting to quantify endogenous protein and track changes after perturbing proteasome activity or E2 partner availability. In immunofluorescence, the antibody highlights nuclear and lamina-associated patterns that can be correlated with chromatin markers or transcriptional co-regulators. Co-immunoprecipitation with RNF220 Antibody enables the capture of complexes for proteomic or ubiquitin-site analysis, helping define substrate spectra under differentiating or stress conditions. In translational studies, measuring Ring finger protein 220 may clarify mechanisms underlying white matter disorders and other developmental phenotypes where ubiquitin signaling is disrupted.

The RNF220 Antibody from NSJ Bioreagents integrates smoothly with ubiquitin pathway workflows, including tandem ubiquitin-binding entity pulldowns, cycloheximide chase assays, and proximity labeling approaches. By combining precise detection with functional readouts of protein half-life and ubiquitin linkage type, investigators can delineate how Ring finger protein 220 balances substrate stabilization and turnover in the nucleus. This antibody is equally at home in discovery pipelines and targeted validation, supporting both atlas-scale perturbation screens and focused mechanistic experiments.

As the field expands its view of how E3 ligases sculpt transcriptional landscapes, RNF220 Antibody offers a reliable handle on an underexplored regulator. Its versatility across imaging, pulldown, and quantitative immunoblotting makes it a cornerstone reagent for decoding how ubiquitin-dependent control of transcription factors and co-regulators shapes lineage choice, adaptation, and disease progression.

UniProt: Q5VTB9