NPLOC4 Antikörper (AA 1-510)

Produktnummer ABIN7869444

Der Kaninchen Polyklonal anti-NPLOC4 Antikörper wird verwendet zum Nachweis von NPLOC4 in Proben von Zebrafisch (Danio rerio). Er wurde validiert für IHC (p).

Kurzübersicht für NPLOC4 Antikörper (AA 1-510) (ABIN7869444)

Target: NPLOC4 (Nuclear Protein Localization 4 Homolog (NPLOC4))

Reaktivität: Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPLOC4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NPLOC4 Antikörper

Bindungsspezifität: AA 1-510

Verwendungszweck: Zebrafish Nploc4 Antibody / Npl4 / Nuclear protein localization protein 4

Aufreinigung: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Nploc4 recombinant protein (amino acids M1-C510) was used as the immunogen for the Zebrafish Nploc4 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Zebrafish Nploc4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Zebrafish Nploc4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NPLOC4

Target: NPLOC4 (Nuclear Protein Localization 4 Homolog (NPLOC4))

Andere Bezeichnung: Nploc4

Hintergrund: Nploc4, or nuclear protein localization protein four homolog, is a highly conserved protein involved in protein degradation and cellular stress response pathways. It acts as a cofactor in the disassembly of polyubiquitinated protein complexes, particularly in cooperation with the ATPase p97 also known as VCP. Nploc4 plays an essential role in extracting ubiquitinated substrates from membranes and protein complexes to facilitate their degradation by the proteasome.

In zebrafish, Nploc4 is expressed in multiple tissues and is especially active in proliferative or metabolically active cells. The zebrafish Nploc4 protein is an ortholog of the human NPLOC4 gene. Both versions of the protein share strong sequence and functional conservation, indicating that zebrafish can serve as a reliable model for studying NPLOC4 related biology.

Functionally, Nploc4 is part of a complex with Ufd1 and p97, and this complex is critical for endoplasmic reticulum associated degradation, chromatin remodeling, and cell cycle regulation. Disruption of Nploc4 function in zebrafish leads to defects in protein homeostasis and developmental abnormalities, especially in systems that are sensitive to proteotoxic stress such as the nervous system and musculature.

Given its central role in maintaining cellular protein quality control and its conservation with human NPLOC4, zebrafish Nploc4 is commonly used in developmental studies and disease models related to protein aggregation, neurodegeneration, and cellular stress adaptation.

UniProt: Q7ZVI8