Rekombinanter Tryptophan Hydroxylase 2 Antikörper (AA 1-490)

Produktnummer ABIN7869410

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Tryptophan Hydroxylase 2 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Maus und Ratte.

Kurzübersicht für Rekombinanter Tryptophan Hydroxylase 2 Antikörper (AA 1-490) (ABIN7869410)

Target: Tryptophan Hydroxylase 2 (TPH2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Tryptophan Hydroxylase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 31T01

Produktdetails anti-Tryptophan Hydroxylase 2 Antikörper

Bindungsspezifität: AA 1-490

Verwendungszweck: TPH2 Antibody / Tryptophan hydroxylase 2

Aufreinigung: Affinity chromatography

Immunogen: Recombinant protein with human THP2 (Position: M1-I490) was used as the immunogen for the TPH2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TPH2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the TPH2 antibody at -20oC.

Details zu Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Andere Bezeichnung: TPH2

Hintergrund: TPH2 antibody detects Tryptophan hydroxylase 2, encoded by the TPH2 gene. Tryptophan hydroxylase 2 is the rate limiting enzyme in the biosynthesis of serotonin in the brain. By catalyzing the hydroxylation of tryptophan to 5 hydroxytryptophan, which is then decarboxylated to serotonin, this enzyme plays an essential role in neurotransmission, mood regulation, and circadian rhythms. TPH2 antibody provides researchers with a vital tool to study serotonin metabolism, neuronal signaling, and psychiatric disorders.

Tryptophan hydroxylase 2 is distinct from TPH1, which performs the same enzymatic reaction but is expressed primarily in peripheral tissues. TPH2 is enriched in serotonergic neurons of the raphe nuclei in the brainstem. Research with TPH2 antibody has revealed that disruption of this enzyme reduces central serotonin production, leading to alterations in behavior, mood, and stress responses. This central role has made TPH2 a focus of studies in depression, anxiety, and other mood disorders.

Mutations in TPH2 have been associated with psychiatric disease and developmental disorders. Genetic variants that reduce enzyme activity have been linked to susceptibility to depression, bipolar disorder, and autism spectrum disorders. Studies using TPH2 antibody in postmortem brain tissue have confirmed altered expression in individuals with psychiatric conditions, supporting its clinical relevance. Animal models with disrupted TPH2 expression also display behavioral phenotypes, reinforcing the causal role of this enzyme in serotonin dependent pathways.

Beyond psychiatry, TPH2 is relevant in circadian biology, stress physiology, and drug response. Serotonin produced by TPH2 serves as a precursor for melatonin synthesis in the pineal gland, linking it to circadian regulation. Research with TPH2 antibody has shown that stress hormones modulate its expression, suggesting integration of environmental cues with neurotransmitter metabolism. Because many antidepressants and antipsychotics target serotonin pathways, TPH2 remains an important pharmacological research target.

TPH2 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting measures enzyme levels in brain lysates, immunohistochemistry demonstrates expression in raphe nuclei and other serotonergic structures, and immunofluorescence allows visualization of neuronal localization. These methods make TPH2 antibody indispensable in neuroscience research.

By providing validated TPH2 antibody reagents, NSJ Bioreagents supports research into neurotransmitter metabolism, brain development, and psychiatric disease. Detection of Tryptophan hydroxylase 2 provides researchers with a reliable means to study serotonin pathways in health and disease.

UniProt: Q8IWU9