LYAR Antikörper (AA 1-379)

Produktnummer ABIN7869273

Der Kaninchen Polyklonal anti-LYAR Antikörper wird verwendet zum Nachweis von LYAR in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IP, ICC und IF.

Kurzübersicht für LYAR Antikörper (AA 1-379) (ABIN7869273)

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYAR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-LYAR Antikörper

Bindungsspezifität: AA 1-379

Verwendungszweck: LYAR Antibody / Cell growth-regulating nucleolar protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LYAR recombinant protein (Position: M1-K379) was used as the immunogen for the LYAR antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LYAR antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LYAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LYAR

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Andere Bezeichnung: LYAR

Substanzklasse: Antibody

Hintergrund: The LYAR antibody targets Cell growth-regulating nucleolar protein, a zinc finger nucleolar protein encoded by the LYAR gene. Originally identified in human leukemia cells, LYAR plays roles in ribosome biogenesis, rRNA processing, and cell proliferation. Cell growth-regulating nucleolar protein associates with pre-ribosomal complexes and contributes to ribosomal subunit maturation and export. The LYAR antibody provides an essential tool for investigating nucleolar function, ribosome assembly, and growth regulation in both normal and transformed cells.

Cell growth-regulating nucleolar protein contains a C2HC-type zinc finger motif and a nuclear localization signal, directing it to the nucleolus where ribosome synthesis occurs. It participates in the early stages of 60S ribosomal subunit formation by binding pre-rRNA and ribonucleoprotein complexes. The LYAR antibody enables detection of this nucleolar marker, supporting studies into the molecular events that control ribosome production and cellular growth rates. Its expression correlates with proliferative activity in rapidly dividing cells.

LYAR has emerged as a multifunctional protein involved in transcriptional regulation and cell cycle progression. It interacts with nucleolin and other nucleolar proteins to coordinate ribosomal RNA processing. Beyond ribosome synthesis, Cell growth-regulating nucleolar protein influences p53 signaling and apoptosis by modulating nucleolar stress responses. The LYAR antibody supports analysis of these pathways, helping define how nucleolar integrity links to cell cycle checkpoints and stress-induced growth arrest.

Overexpression of LYAR has been observed in several cancers, including colorectal, prostate, and hepatocellular carcinoma. It promotes cell proliferation and survival by enhancing rRNA synthesis and suppressing apoptosis. The LYAR antibody enables quantitative assessment of its expression in tumor tissues and cell models, aiding investigation into oncogenic mechanisms involving ribosome biogenesis. In contrast, depletion of LYAR impairs ribosomal maturation and reduces cell growth, underscoring its importance for proliferation control.

The LYAR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing distinct nucleolar staining consistent with its localization. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for use in cell biology, cancer research, and molecular genetics. By supporting precise detection of Cell growth-regulating nucleolar protein, the LYAR antibody advances understanding of ribosome biogenesis, nucleolar stress, and their contributions to tumorigenesis.

UniProt: Q9NX58