Sideroflexin 4 Antikörper (AA 1-337)

Produktnummer ABIN7869215

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Sideroflexin 4 in ELISA, WB, IF, IHC, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Sideroflexin 4 Antikörper (AA 1-337) (ABIN7869215)

Target: Sideroflexin 4 (SFXN4)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sideroflexin 4 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Sideroflexin 4 Antikörper

Bindungsspezifität: AA 1-337

Verwendungszweck: SFXN4 Antibody / Sideroflexin 4

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SFXN4 recombinant protein (Position: M1-V337) was used as the immunogen for the SFXN4 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SFXN4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SFXN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu Sideroflexin 4

Target: Sideroflexin 4 (SFXN4)

Andere Bezeichnung: SFXN4

Hintergrund: SFXN4 antibody detects Sideroflexin-4, a mitochondrial inner membrane protein involved in iron-sulfur cluster biogenesis and mitochondrial respiration. The UniProt recommended name is Sideroflexin-4 (SFXN4), a member of the sideroflexin family of transporters that regulate iron metabolism and mitochondrial energy production. SFXN4 plays a crucial role in maintaining mitochondrial homeostasis by supporting the synthesis and incorporation of iron-sulfur cofactors into respiratory chain complexes.

Functionally, SFXN4 antibody identifies a 317-amino-acid transmembrane protein that contributes to the maturation of mitochondrial iron-sulfur (Fe-S) proteins. SFXN4 facilitates iron import into the mitochondrial matrix and interacts with key components of the Fe-S assembly machinery, including ISCU and NFS1. Through this interaction, it ensures the proper incorporation of Fe-S clusters into enzymes critical for electron transport, oxidative phosphorylation, and metabolic regulation. Disruption of SFXN4 impairs mitochondrial respiration, leading to decreased ATP production and increased oxidative stress.

The SFXN4 gene is located on chromosome 10q26.3 and encodes a protein with multiple transmembrane domains localized to the inner mitochondrial membrane. It belongs to the sideroflexin family, which includes five human homologs (SFXN1-SFXN5) with distinct but overlapping roles in amino acid and iron transport. Among these, SFXN4 has a specialized role in mitochondrial iron utilization and heme biosynthesis. Loss-of-function mutations in SFXN4 cause mitochondrial complex I and III deficiencies, resulting in mitochondrial myopathy, anemia, and developmental delay.

In normal physiology, SFXN4 maintains iron homeostasis and supports mitochondrial protein synthesis. It is essential for cell survival under metabolic stress conditions requiring efficient oxidative phosphorylation. Dysfunction of SFXN4 affects the activity of Fe-S cluster-dependent enzymes such as aconitase and succinate dehydrogenase, contributing to defects in the tricarboxylic acid (TCA) cycle and respiratory chain function.

SFXN4 antibody is widely used in mitochondrial biology, metabolism, and bioenergetics research. It is valuable for western blotting, immunofluorescence, and mitochondrial fractionation studies to analyze SFXN4 expression and localization. In disease research, this antibody helps investigate mitochondrial dysfunctions linked to anemia, neurodegeneration, and metabolic syndromes. Reduced SFXN4 expression is associated with impaired mitochondrial translation and decreased oxidative capacity, while overexpression may alter cellular redox balance.

Structurally, SFXN4 contains multiple hydrophobic helices forming a membrane-spanning topology that mediates metabolite and ion transport. Its function depends on coordination with mitochondrial carrier proteins and iron chaperones. NSJ Bioreagents provides SFXN4 antibody reagents validated for use in mitochondrial metabolism, iron homeostasis, and respiratory chain research.

UniProt: Q6P4A7

Pathways: Transition Metal Ion Homeostasis