TRIM44 Antikörper (AA 1-332)

Produktnummer ABIN7869203

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TRIM44 in WB, ELISA und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für TRIM44 Antikörper (AA 1-332) (ABIN7869203)

Target: TRIM44 (Tripartite Motif Containing 44 (TRIM44))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRIM44 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-TRIM44 Antikörper

Bindungsspezifität: AA 1-332

Verwendungszweck: TRIM44 Antibody / Tripartite motif-containing protein 44

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TRIM44 recombinant protein (Position: M1-E332) was used as the immunogen for the TRIM44 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TRIM44 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TRIM44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TRIM44

Target: TRIM44 (Tripartite Motif Containing 44 (TRIM44))

Andere Bezeichnung: TRIM44

Hintergrund: TRIM44 antibody detects Tripartite motif-containing protein 44, an E3 ubiquitin ligase-like protein that regulates cell growth, stress response, and signaling through its role in ubiquitination and protein stabilization. Encoded by the TRIM44 gene on chromosome 11p13, this protein belongs to the tripartite motif (TRIM) family, characterized by a RING finger domain, B-box motifs, and a coiled-coil region that mediate protein-protein interactions. TRIM44 functions as a scaffold protein that influences autophagy, signal transduction, and oncogenic pathways by regulating substrate ubiquitination.

Structurally, TRIM44 is a 512-amino-acid cytoplasmic protein of approximately 57 kilodaltons containing a zinc-binding B-box and coiled-coil domains typical of TRIM proteins. Unlike many family members, TRIM44 lacks a canonical RING finger domain but instead contains a zinc metalloprotease-like region that mediates protein stabilization rather than degradation. This structural divergence enables TRIM44 to protect target proteins from proteasomal degradation, thereby modulating multiple signaling cascades including NF-?B and PI3K/AKT pathways.

The TRIM44 antibody is widely used in cancer biology, immunology, and cell signaling research to study ubiquitin-dependent protein regulation, tumor progression, and cellular stress response. Western blot analysis detects a 57 kilodalton band corresponding to TRIM44, while immunofluorescence shows cytoplasmic and perinuclear staining, reflecting its role in signaling and vesicular trafficking. This antibody provides a dependable reagent for investigating the molecular mechanisms by which TRIM44 regulates protein turnover and signal transduction.

Functionally, TRIM44 stabilizes key signaling proteins including mTOR and NF-?B regulators, promoting survival under stress conditions such as oxidative damage or nutrient deprivation. Overexpression of TRIM44 has been observed in multiple cancers including gastric, lung, and breast carcinomas, where it enhances proliferation, migration, and epithelial-mesenchymal transition (EMT). Conversely, its depletion sensitizes cells to apoptosis and autophagic degradation. The TRIM44 antibody supports research exploring ubiquitin signaling networks, autophagy regulation, and cancer-associated proteostasis. NSJ Bioreagents validates this antibody for its applications ensuring reproducible performance in cellular signaling and oncogenic pathway studies.

UniProt: Q96DX7