NOXO1 Antikörper (AA 1-294)

Produktnummer ABIN7869065

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NOXO1 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für NOXO1 Antikörper (AA 1-294) (ABIN7869065)

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOXO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-NOXO1 Antikörper

Bindungsspezifität: AA 1-294

Verwendungszweck: NOXO1 Antibody / NADPH oxidase organizer 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NOXO1 recombinant protein (Position: M1-E294) was used as the immunogen for the NOXO1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NOXO1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NOXO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NOXO1

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Andere Bezeichnung: NOXO1

Hintergrund: NOXO1 antibody detects NADPH oxidase organizer 1, a cytosolic adaptor protein that regulates reactive oxygen species (ROS) production by the NADPH oxidase complex. The UniProt recommended name is NADPH oxidase organizer 1 (NOXO1). This protein is a functional homolog of p47phox and is required for assembling and activating NADPH oxidase in nonphagocytic cells.

Functionally, NOXO1 antibody identifies a 376-amino-acid protein containing PX (phox homology) and SH3 (Src homology 3) domains that mediate membrane localization and protein-protein interactions. NOXO1 binds to membrane subunits such as NOX1, NOXA1, and p22phox, organizing them into an active complex that transfers electrons from NADPH to oxygen, generating superoxide. This process supports cellular signaling, host defense, and redox homeostasis.

The NOXO1 gene is located on chromosome 16q22.1 and encodes a regulatory component expressed in epithelial cells of the colon, prostate, and vascular tissues. Unlike its phagocytic counterpart p47phox, NOXO1 lacks an autoinhibitory region, resulting in constitutive activity under resting conditions. Its expression enables sustained ROS generation in nonimmune cells for physiological signaling functions such as cell proliferation and differentiation.

In redox signaling, NOXO1-derived ROS modulate kinase activation, ion transport, and gene expression. In the intestine, NOXO1 contributes to mucosal defense and microbial balance by supporting NOX1-mediated oxidative activity. In vascular and neural tissues, it influences smooth muscle tone and neuronal excitability. Dysregulation of NOXO1 expression or complex assembly has been implicated in oxidative stress-related disorders, including inflammatory bowel disease and hypertension.

NOXO1 antibody is widely used in redox biology, signaling, and oxidative stress research. It is suitable for western blotting, immunofluorescence, and co-immunoprecipitation to analyze NOXO1 expression and complex formation. This antibody aids in studying NADPH oxidase regulation, reactive oxygen generation, and oxidative signaling pathways. It is also useful in exploring NOX1 and NOX3 complex activity in epithelial and neuronal systems.

Structurally, NOXO1 features an N-terminal PX domain that binds phosphoinositides and C-terminal SH3 motifs that engage proline-rich regions of target proteins. Post-translational regulation includes phosphorylation and redox-dependent modifications that modulate its interaction with NOXA1 and NOX1. NSJ Bioreagents provides NOXO1 antibody reagents validated for use in redox signaling, ROS metabolism, and membrane complex assembly studies.

UniProt: Q8NFA2