POLR1E Antikörper (AA 1-291)

Produktnummer ABIN7869059

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch POLR1E in WB, ELISA, IP, IF und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für POLR1E Antikörper (AA 1-291) (ABIN7869059)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR1E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-POLR1E Antikörper

Bindungsspezifität: AA 1-291

Verwendungszweck: POLR1E Antibody / DNA-directed RNA polymerase I subunit E / PAF53

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) was used as the immunogen for the POLR1E antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the POLR1E antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the POLR1E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Andere Bezeichnung: POLR1E

Hintergrund: POLR1E antibody detects DNA-directed RNA polymerase I subunit E (also known as PAF53), a core component of the RNA polymerase I complex responsible for synthesizing ribosomal RNA (rRNA) precursors. Encoded by the POLR1E gene on chromosome 9q34.11, this protein is essential for rRNA transcription and ribosome biogenesis. POLR1E interacts with other polymerase I subunits, transcription initiation factors, and nucleolar scaffolding proteins to regulate transcription of the 47S rRNA gene cluster. By supporting nucleolar organization and transcriptional efficiency, it plays a fundamental role in cell growth and proliferation.

POLR1E localizes to the nucleolus and associates with the transcription initiation factor SL1 and upstream binding factor (UBF) to form a preinitiation complex at rDNA promoters. Its C-terminal domain mediates binding to RNA polymerase I subunit RPA1 and other core enzyme components, facilitating enzyme assembly and promoter clearance. Loss of POLR1E disrupts nucleolar architecture and reduces rRNA synthesis, leading to impaired ribosome production and cell-cycle arrest. This makes it a critical determinant of nucleolar integrity and growth control.

The POLR1E antibody is widely used in molecular biology, cancer research, and nucleolar studies to assess RNA polymerase I activity and rRNA transcription regulation. Western blot analysis identifies a 53 kilodalton band corresponding to POLR1E, while immunofluorescence reveals punctate nucleolar staining. Because RNA polymerase I transcription drives ribosome production, alterations in POLR1E expression are linked to tumor growth and metabolic adaptation. Increased expression has been observed in rapidly dividing cells, whereas downregulation occurs under cellular stress or growth inhibition.

In addition to its role in transcription, POLR1E contributes to the nucleolar stress response by coordinating signaling between rRNA synthesis and p53 activation. Defects in polymerase I assembly or activity trigger nucleolar stress pathways that stabilize p53, resulting in growth arrest. The POLR1E antibody enables exploration of these mechanisms and supports investigations into ribosomopathies, tumorigenesis, and cellular aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise and reliable detection across model systems.

UniProt: Q9GZS1