NOTO Antikörper (AA 1-241)

Produktnummer ABIN7868905

Der Kaninchen Polyklonal anti-NOTO Antikörper (ABIN7868905) detektiert spezifisch NOTO in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Zebrafisch (Danio rerio).

Kurzübersicht für NOTO Antikörper (AA 1-241) (ABIN7868905)

Target: NOTO (Notochord Homeobox (NOTO))

Reaktivität: Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOTO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NOTO Antikörper

Bindungsspezifität: AA 1-241

Verwendungszweck: Zebrafish Noto Antibody / Notochord homeobox protein / Floating head

Aufreinigung: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Noto recombinant protein (amino acids M1-D241) was used as the immunogen for the Zebrafish Noto antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Zebrafish Noto antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the Zebrafish Noto antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NOTO

Target: NOTO (Notochord Homeobox (NOTO))

Andere Bezeichnung: Noto

Hintergrund: Noto, or notochord homeobox protein, is a transcription factor that plays a fundamental role in the formation and patterning of the notochord and tailbud during early embryonic development. In zebrafish, Noto is essential for proper axis formation, neural tube development, and left right asymmetry.

Zebrafish Noto is considered the functional ortholog of the human NOTO gene. Both zebrafish and human versions of this gene encode homeodomain containing transcription factors that are critical for notochord morphogenesis and cilia driven fluid flow, which influences organ laterality during development. Despite some sequence divergence between species, the developmental roles of zebrafish Noto and human NOTO are highly conserved.

In the zebrafish embryo, Noto is expressed in the dorsal organizer, the notochordal plate, and the developing tailbud. It regulates genes involved in axial mesoderm formation and ciliogenesis, and it interacts with other developmental signals including components of the nodal and hedgehog pathways. Loss of Noto function leads to defects in notochord structure, tail elongation, and laterality, including reversed heart and gut looping.

Because of its critical role in vertebrate axis and notochord formation, zebrafish Noto is widely used in developmental biology to study early mesodermal differentiation, organ positioning, and congenital disorders related to axis specification. Its functional conservation with the human NOTO gene also makes it a useful model for investigating the genetic basis of developmental syndromes affecting the spine and body plan.

UniProt: Q90461