NUDT8 Antikörper (AA 1-236)

Produktnummer ABIN7868890

Der Kaninchen Polyklonal anti-NUDT8 Antikörper (ABIN7868890) detektiert spezifisch NUDT8 in WB, IHC, FACS und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NUDT8 Antikörper (AA 1-236) (ABIN7868890)

Target: NUDT8 (Nudix Hydrolase 8 (NUDT8))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUDT8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Produktdetails anti-NUDT8 Antikörper

Bindungsspezifität: AA 1-236

Verwendungszweck: NUDT8 Antibody / Nudix hydrolase 8

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NUDT8 recombinant protein (Position: M1-L236) was used as the immunogen for the NUDT8 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NUDT8 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NUDT8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NUDT8

Target: NUDT8 (Nudix Hydrolase 8 (NUDT8))

Andere Bezeichnung: NUDT8

Hintergrund: NUDT8 antibody detects Nudix hydrolase 8, a mitochondrial enzyme encoded by the NUDT8 gene on chromosome 4p14. NUDT8 belongs to the Nudix hydrolase family of pyrophosphatases, which catalyze the hydrolysis of nucleoside diphosphates and related metabolites to prevent accumulation of potentially toxic intermediates. NUDT8 is localized to the mitochondrial matrix, where it plays a role in maintaining nucleotide balance and mitochondrial integrity. It is expressed in energy-demanding tissues such as heart, skeletal muscle, and brain, reflecting its role in nucleotide metabolism and redox regulation.

Structurally, NUDT8 contains the conserved Nudix (nucleoside diphosphate linked to another moiety, X) motif GX5EX7REUXEEXGU, which defines the catalytic site of the Nudix hydrolase family. The enzyme hydrolyzes substrates such as CoA diphosphate derivatives, ADP-ribose, and other nucleotide intermediates. NUDT8 belongs to the mitochondrial subfamily of Nudix enzymes, which also includes NUDT9 and NUDT15. Co-localization studies confirm its mitochondrial matrix localization, consistent with roles in energy metabolism and oxidative stress defense.

Functionally, NUDT8 acts as a metabolic housekeeper by removing oxidized or excess nucleotide derivatives that can disrupt mitochondrial function. It maintains CoA and ADP-ribose homeostasis, supporting efficient energy production and mitochondrial redox balance. NUDT8 also contributes to nucleotide quality control by hydrolyzing damaged substrates generated during oxidative stress. Through this activity, it supports mitochondrial DNA replication fidelity and prevents accumulation of noncanonical nucleotides.

NUDT8 expression is developmentally regulated, increasing during mitochondrial biogenesis and metabolic activation in muscle and neuronal cells. Dysregulation of NUDT8 has been linked to metabolic disorders and mitochondrial dysfunction. Reduced enzyme activity can impair oxidative phosphorylation, while overexpression enhances resilience against oxidative damage. Pathway involvement includes CoA metabolism, NAD metabolism, and nucleotide salvage pathways.

The NUDT8 antibody from NSJ Bioreagents is a valuable reagent for research into mitochondrial metabolism, nucleotide regulation, and redox homeostasis.

UniProt: Q8WV74