GAMT Antikörper (AA 1-236)

Produktnummer ABIN7868889

Der Kaninchen Polyklonal anti-GAMT Antikörper wird verwendet zum Nachweis von GAMT in Proben von Human, Ratte und Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für GAMT Antikörper (AA 1-236) (ABIN7868889)

Target: GAMT (Guanidinoacetate N-Methyltransferase (GAMT))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-GAMT Antikörper

Bindungsspezifität: AA 1-236

Verwendungszweck: GAMT Antibody / Guanidinoacetate N-methyltransferase

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human GAMT recombinant protein (Position: M1-G236) was used as the immunogen for the GAMT antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GAMT antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GAMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GAMT

Target: GAMT (Guanidinoacetate N-Methyltransferase (GAMT))

Andere Bezeichnung: GAMT

Hintergrund: GAMT antibody detects Guanidinoacetate N-methyltransferase, an enzyme that catalyzes the final step in the biosynthesis of creatine, a key molecule for cellular energy storage and transfer. GAMT converts guanidinoacetate and S-adenosyl-L-methionine into creatine and S-adenosyl-L-homocysteine, a critical reaction for maintaining high-energy phosphate metabolism in muscle and neural tissues. The GAMT antibody is widely used in metabolism, neurology, and biochemical research to study creatine biosynthesis, methylation pathways, and energy regulation.

GAMT is encoded by the GAMT gene located on human chromosome 19p13.3. The protein is approximately 236 amino acids long and localizes to the cytoplasm and mitochondria of metabolically active cells such as skeletal muscle fibers, hepatocytes, and neurons. GAMT belongs to the family of methyltransferases and functions as a homodimer, with each subunit contributing to catalytic activity and substrate specificity.

The GAMT antibody detects a 28 kilodalton protein by western blot and shows cytosolic and mitochondrial localization by immunofluorescence microscopy. Creatine synthesized by GAMT is essential for buffering cellular ATP levels through the phosphocreatine shuttle system, particularly in tissues with fluctuating energy demands. In the brain, creatine supports neuronal excitability and synaptic transmission, while in muscle, it sustains rapid contraction and recovery.

Deficiency of GAMT causes guanidinoacetate methyltransferase deficiency, a rare inborn error of metabolism characterized by creatine depletion in the brain, developmental delay, seizures, and movement disorders. Accumulation of guanidinoacetate is neurotoxic, leading to oxidative stress and neurotransmission imbalance. Early diagnosis through enzyme assays or molecular testing is critical, as dietary creatine supplementation can mitigate neurological symptoms.

Beyond its metabolic role, GAMT activity influences methylation capacity, homocysteine metabolism, and nitric oxide synthesis, linking it to broader aspects of cellular function. Altered GAMT expression has been associated with cardiovascular disease, neurodegeneration, and aging due to impaired energy homeostasis and methyl donor imbalance.

Because GAMT is central to energy metabolism and methyl group regulation, it serves as an important biomarker for studying metabolic diseases and neurological function. NSJ Bioreagents provides a validated GAMT antibody optimized for its applications, supporting research into creatine metabolism, mitochondrial function, and energy regulation.

UniProt: Q14353