Myosin Antikörper (AA 1-1895)

Produktnummer ABIN7868329

Der Kaninchen Polyklonal anti-Myosin Antikörper (ABIN7868329) detektiert spezifisch Myosin in WB, ELISA, IHC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Myosin Antikörper (AA 1-1895) (ABIN7868329)

Target: Myosin

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myosin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Myosin Antikörper

Bindungsspezifität: AA 1-1895

Verwendungszweck: MYH14 Antibody / Myosin 14

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MYH14 recombinant protein (Position: M1-R1895) was used as the immunogen for the MYH14 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MYH14 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MYH14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu Myosin

Target: Myosin

Andere Bezeichnung: MYH14

Hintergrund: MYH14 antibody detects Myosin-14, encoded by the MYH14 gene on chromosome 19q13.33. MYH14 antibody is used in studies of cytoskeletal regulation, hearing, and cellular motility. Myosin-14 belongs to the class II non-muscle myosins, functioning as an actin-based motor protein. It regulates actin filament organization, adhesion, and vesicle trafficking. Expression is widespread, with high levels in inner ear hair cells, smooth muscle, and neuronal tissue, where it supports specialized mechanical and sensory functions.

Structurally, Myosin-14 is a large protein containing an N-terminal motor domain with ATPase activity, a neck region with IQ motifs that bind light chains, and a coiled-coil tail that mediates dimerization and filament assembly. Its motor domain hydrolyzes ATP to generate force for actin filament sliding, supporting contractility and cytoskeletal rearrangements. Isoforms derived from alternative splicing confer tissue-specific functions.

Functionally, Myosin-14 regulates actin cytoskeleton dynamics, contributing to cell migration, adhesion, and intracellular transport. In neurons, it supports axon guidance and synaptic function. In the inner ear, Myosin-14 is essential for mechanotransduction and hearing. Knockout or mutation of MYH14 disrupts hair cell function, leading to progressive hearing loss. Researchers use MYH14 antibody to investigate cytoskeletal biology, neuronal function, and auditory physiology.

Clinically, MYH14 mutations are associated with autosomal dominant non-syndromic hearing loss (DFNA4A). Mutations disrupt motor activity or actin binding, impairing hair cell function. Myosin-14 variants are also linked to peripheral neuropathy and craniofacial developmental disorders. Altered expression of MYH14 has been observed in cancers, where cytoskeletal regulation influences invasion and metastasis. NSJ Bioreagents offers MYH14 antibody as a tool for studies of cytoskeletal regulation, auditory biology, and disease mechanisms.

Experimentally, MYH14 antibody is used in western blotting to detect the ~230 kDa protein, in immunofluorescence microscopy to visualize actin-associated localization, and in immunohistochemistry to study hair cell and neuronal expression. Co-immunoprecipitation with MYH14 antibody isolates complexes of actin, myosin light chains, and regulatory proteins.

UniProt: Q7Z406