SPA17 Antikörper (AA 1-136)

Produktnummer ABIN7868130

Der Kaninchen Polyklonal anti-SPA17 Antikörper wird verwendet zum Nachweis von SPA17 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für SPA17 Antikörper (AA 1-136) (ABIN7868130)

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPA17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-SPA17 Antikörper

Bindungsspezifität: AA 1-136

Verwendungszweck: SPA17 Antibody / Sperm autoantigenic protein 17

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SPA17 recombinant protein (Position: M1-K136) was used as the immunogen for the SPA17 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SPA17 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SPA17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SPA17

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Andere Bezeichnung: SPA17

Hintergrund: SPA17 antibody recognizes Sperm autoantigenic protein 17, a testis-enriched protein encoded by the SPA17 gene that is involved in sperm motility, fertilization, and cell adhesion. SPA17 is expressed primarily in the acrosomal region of mature spermatozoa and in ciliated epithelial tissues, where it contributes to cell-cell interaction and immune recognition processes. The protein contains an N-terminal sperm-coating antigen domain and a C-terminal region homologous to the heat shock protein family, suggesting a chaperone-like role in maintaining sperm surface protein stability.

SPA17 was initially identified as an autoantigen in patients with infertility and autoimmune disorders, reflecting its immunogenic nature. It is also classified as a cancer-testis antigen due to its restricted normal expression in testicular tissue and aberrant expression in various malignancies, including ovarian, prostate, and lung cancers. This re-expression in tumors has made SPA17 a target of interest for cancer immunotherapy and tumor biomarker research. Functionally, SPA17 promotes sperm-egg interaction by binding to carbohydrate residues on the zona pellucida and may participate in intracellular signaling during acrosomal exocytosis.

The SPA17 gene is located on chromosome 11q24.2 and encodes a 151-amino acid protein that forms homodimers. The protein associates with the outer acrosomal membrane and flagellar structures, supporting motility and fertilization capacity. Beyond reproduction, SPA17 has been detected in immune and neural tissues, where it may modulate cAMP-mediated signaling pathways. Studies have shown SPA17 interaction with protein kinase A (PKA) regulatory subunits, linking it to cellular adhesion and signal transduction.

Immunohistochemical analysis using SPA17 antibody reveals strong staining in testis, epididymis, and certain tumor tissues. The antibody is widely used to investigate sperm biology, fertility disorders, and tumor immunogenicity. SPA17 antibody from NSJ Bioreagents provides a reliable reagent for studying reproductive protein function and cancer-testis antigen expression in diverse research applications

UniProt: Q15506