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IKK-alpha /IKK-beta Antikörper (pSer176, pSer180)

Der Rabbit Polyclonal Anti-IKK-alpha /IKK-beta-Antikörper wurde für WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) validiert. Er ist geeignet, IKK-alpha /IKK-beta in Proben von Mouse, Human zu detektieren. Es sind 2+ Publikationen verfügbar.
Produktnummer ABIN743318

Kurzübersicht für IKK-alpha /IKK-beta Antikörper (pSer176, pSer180) (ABIN743318)

Target

IKK-alpha /IKK-beta

Reaktivität

  • 73
  • 47
  • 28
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Maus, Human

Wirt

  • 78
Kaninchen

Klonalität

  • 69
  • 9
Polyklonal

Konjugat

  • 22
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser IKK-alpha /IKK-beta Antikörper ist unkonjugiert

Applikation

  • 49
  • 40
  • 39
  • 21
  • 15
  • 12
  • 9
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 34
    • 24
    • 18
    • 15
    • 10
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer176, pSer180

    Spezifität

    These phosphorylation sites are homologous across the listed species.

    Kreuzreaktivität

    Human, Maus

    Homologie

    Rat,Dog,Cow,Pig,Horse,Chicken

    Aufreinigung

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).

    Gao, Guo, Wang, Lin, Yu, Sheng, Wu, Gong: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." in: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).

  • Target

    IKK-alpha /IKK-beta

    Andere Bezeichnung

    IKK alpha/beta /

    Hintergrund

    Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16, TCF-16, CHUK

    Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

    Gen-ID

    1147

    UniProt

    O15111
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