VEGF Antikörper

Produktnummer ABIN7865754

Der Maus Monoklonal anti-VEGF Antikörper wird verwendet zum Nachweis von VEGF in Proben von Human, Maus, Ratte, Hund und Kaninchen. Er wurde validiert für IHC, IF, FACS und ICC.

Kurzübersicht für VEGF Antikörper (ABIN7865754)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: VEGF-21

Produktdetails anti-VEGF Antikörper

Verwendungszweck: anti-Vascular Endothelial Growth Factor (VEGF) mouse monoclonal, VEGF-21, purified

Aufreinigung: affinity chromatography

Immunogen: human VEGF189 recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Hintergrund: VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells. Positive control: Astrocytomas, breast or ovarian carcinomas.