Proliferation Marker Antikörper

Produktnummer ABIN7865722

Der Maus Monoklonal anti-Proliferation Marker Antikörper (Klon IPO-38) (ABIN7865722) detektiert spezifisch Proliferation Marker in IHC, WB, ELISA und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Proliferation Marker Antikörper (ABIN7865722)

Target: Proliferation Marker

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: IPO-38

Produktdetails

Verwendungszweck: anti-Proliferation Marker mouse monoclonal, IPO-38, purified

Aufreinigung: affinity chromatography

Immunogen: spleen cells of a patient with hairy cell leukemia

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Antigendetails

Target: Proliferation Marker

Hintergrund: IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkin’s disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker. Positive control: Raji cells.