MVP Antikörper

Produktnummer ABIN7865689

Der Maus Monoklonal anti-MVP Antikörper (Klon 1032) (ABIN7865689) detektiert spezifisch MVP in WB, IHC, IF, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MVP Antikörper (ABIN7865689)

Target: MVP (Major Vault Protein (MVP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MVP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 1032

Produktdetails anti-MVP Antikörper

Verwendungszweck: anti-Major vault protein (MVP) mouse monoclonal, 1032, purified

Aufreinigung: affinity chromatography

Immunogen: affinity purified nuclear extract proteins

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu MVP

Target: MVP (Major Vault Protein (MVP))

Hintergrund: 1032 Is specific for the major vault protein, a 104-kDa highly conserved protein interacting with estrogen receptor. It is one of a series of four mAbs which recognize different epitopes of the protein. Major vault proteins have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Positive control: MCF-7 or HeLa cells. Breast cancer.