LY75/DEC-205 Antikörper

Produktnummer ABIN7865686

Der Maus Monoklonal anti-LY75/DEC-205 Antikörper (Klon PN-15) (ABIN7865686) detektiert spezifisch LY75/DEC-205 in WB, IHC, FACS, ICC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Affe.

Kurzübersicht für LY75/DEC-205 Antikörper (ABIN7865686)

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reaktivität: Human, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LY75/DEC-205 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: PN-15

Produktdetails anti-LY75/DEC-205 Antikörper

Verwendungszweck: anti-CD205 mouse monoclonal, PN-15, purified

Aufreinigung: affinity chromatography

Immunogen: renal cortical tissue extract

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Andere Bezeichnung: CD205

Hintergrund: PN-15 reacts with a lectin receptor like glycoprotein of 200 kDa (gp200), present in proximal renal tubules and on urothelium. The antigen is carbohydrate in nature. Other normal tissues that display the antigen include breast, parathyroid glands, thymus and epididymis. Among renal carcinomas 93 % of primary and 84 % of metastatic carcinomas are positive. Bladder cancers are also largely positive. Other tumor types include breast cancer, teratocarcinomas and parathyroid adenomas. The antigen, also called DEC-205, was assigned to CD205 at CD workshop VII. In the immune system it can facilitate tolerance to self-antigens through uptake of apoptosis derived material by dendritic cells, which in turn present fragments through MHC II and MHC I, either inducing or repressing immune responses, depending on the nature of concomitant signals. Positive control: Renal carcinoma, bladder carcinoma.