Keratin 7/17 Antikörper
Kurzübersicht für Keratin 7/17 Antikörper (ABIN7865681)
Target
Alle Keratin 7/17 (KRT7/17) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- anti-Keratin 7+17 mouse monoclonal, C-46, purified
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Kreuzreaktivität (Details)
- not with ferret, mouse, rabbit, rat
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Aufreinigung
- affinity chromatography
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Immunogen
- purified human keratin 7, isolated from HeLa cytoskeleton
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Isotyp
- IgG1 kappa
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Applikationshinweise
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment and/ or pepsin digestion recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- store at 2 - 8 °C
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- Keratin 7/17 (KRT7/17)
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Andere Bezeichnung
- Keratin 7+17
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Hintergrund
- C-46 reacts with keratins 7 (54 kDa) and 17 (46 kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia. Positive control: Relevant simple epithelia like ovary, lung, and breast.
Target
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