Keratin 5/14 Antikörper

Produktnummer ABIN7865680

Der Maus Monoklonal anti-Keratin 5/14 Antikörper (Klon EBS-IF-001) (ABIN7865680) detektiert spezifisch Keratin 5/14 in IHC, ICC, IF, FACS und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Cat.

Kurzübersicht für Keratin 5/14 Antikörper (ABIN7865680)

Target: Keratin 5/14 (KRT5/14)

Reaktivität: Human, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 5/14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Klon: EBS-IF-001

Produktdetails anti-Keratin 5/14 Antikörper

Verwendungszweck: anti-Keratin 5+14 mouse monoclonal, EBS-IF-001, purified

Aufreinigung: affinity chromatography

Immunogen: cytokeratin-enriched extract of feline tongue epithelium

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

WB_dilution: assay dependent

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu Keratin 5/14

Target: Keratin 5/14 (KRT5/14)

Andere Bezeichnung: Keratin 5+14

Hintergrund: EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. Positive control: Tongue.