KRT14 Antikörper (C-Term)
Kurzübersicht für KRT14 Antikörper (C-Term) (ABIN7865678)
Target
Alle KRT14 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 459-472, C-Term
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Verwendungszweck
- anti-Keratin 14 mouse monoclonal, EBS-IF-004, purified
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Aufreinigung
- affinity chromatography
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Immunogen
- AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH
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Isotyp
- IgM kappa
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Applikationshinweise
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- store at 2 - 8 °C
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- KRT14 (Keratin 14 (KRT14))
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Andere Bezeichnung
- Keratin 14
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Hintergrund
- EBS-IF-004 reacts specifically with keratin 14 (50 kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Positive control: Skin.
Target
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