Histone H1 Antikörper
Kurzübersicht für Histone H1 Antikörper (ABIN7865649)
Target
Alle Histone H1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Verwendungszweck
- anti-Histone H1 mouse monoclonal, AE-4, purified
-
Aufreinigung
- affinity chromatography
-
Immunogen
- human leukemia biopsy cells
-
Isotyp
- IgG2a kappa
-
-
-
-
Applikationshinweise
-
ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 100 μg/mL
-
Buffer
- PBS with 0.02 % sodium azide
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C
-
Informationen zur Lagerung
- store at 2 - 8 °C
-
-
- Histone H1
-
Hintergrund
- AE-4 recognizes histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Positive control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma or tonsil.
Target
-