alpha Fetoprotein Antikörper
Kurzübersicht für alpha Fetoprotein Antikörper (ABIN7865556)
Target
Alle alpha Fetoprotein (AFP) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- anti-Alpha fetoprotein (AFP) mouse monoclonal, C3, purified
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Kreuzreaktivität (Details)
- not with cow, mouse, rat
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Aufreinigung
- affinity chromatography
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Immunogen
- alpha fetoprotein isolated from serum of hepatoma patients
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Isotyp
- IgG2a kappa
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Applikationshinweise
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- store at 2 - 8 °C
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Andere Bezeichnung
- Alpha fetoprotein (AFP)
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Hintergrund
- C3 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nunstad to a group of antibodies with low affinity to human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa and expressed in fetal liver but normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some other germ cell tumors and in hepatocellular carcinomas. Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.
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Pathways
- C21-Steroid Hormone Metabolic Process
Target
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