TCR alpha Antikörper

Produktnummer ABIN7845274

Der Maus Monoklonal anti-TCR alpha Antikörper wird verwendet zum Nachweis von TCR alpha in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für TCR alpha Antikörper (ABIN7845274)

Target: TCR alpha (TRA) (T Cell Receptor alpha Locus (TRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TCR alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 6B11

Produktdetails anti-TCR alpha Antikörper

Verwendungszweck: Mouse monoclonal antibody to TCR V alpha 24-J alpha 18

Spezifität: Recognizes human TCR V alpha 24-J alpha 18

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Peptide epitope in the CDR3 region of V alpha 24

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu TCR alpha

Target: TCR alpha (TRA) (T Cell Receptor alpha Locus (TRA))

Andere Bezeichnung: TRA

Hintergrund: INKT cell, iNKT, invariant natural killer T cel, invariant NK T cell, invariant NKT cell

Gen-ID: 6955