LY6 Antikörper (Pacific Blue)

Produktnummer ABIN7840915

Der Ratte Monoklonal anti-LY6 Antikörper wird verwendet zum Nachweis von LY6 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für LY6 Antikörper (Pacific Blue) (ABIN7840915)

Target: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LY6 Antikörper ist konjugiert mit Pacific Blue

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: D7

Produktdetails anti-LY6 Antikörper (Pacific Blue)

Verwendungszweck: Rat monoclonal antibody Pacific Blue labled to Ly-6A/E

Spezifität: Recognizes mouse Ly-6A/E

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu LY6

Target: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Andere Bezeichnung: Ly6

Hintergrund: Ly6, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly67, Sca-2, Tsa-1, Lymphocyte antigen 6E, Ly-6E, Stem cell antigen 2, Thymic shared antigen 1, TSA-1

Gen-ID: 110454