LY-49I (LY-49I) Antikörper (FITC)

Produktnummer ABIN7840911

Dieser Maus Monoklonal Antikörper detektiert spezifisch LY-49I in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für LY-49I (LY-49I) Antikörper (FITC) (ABIN7840911)

Target: LY-49I

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: YLI-90

Produktdetails

Verwendungszweck: Mouse monoclonal antibody FITC labled to Ly-49I

Spezifität: Recognizes mouse Ly-49I

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Antigendetails

Target: LY-49I

Andere Bezeichnung: Ly49I

Hintergrund: MHC class I recognition receptor Ly49I, Natural killer cell receptor Ly49C

Gen-ID: 16640