Ki-67 Antikörper (FITC)

Produktnummer ABIN7840347

Dieser Maus Monoklonal Antikörper detektiert spezifisch Ki-67 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Ki-67 Antikörper (FITC) (ABIN7840347)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ki-67 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 3D5

Produktdetails anti-Ki-67 Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to Ki67

Spezifität: Recognizes human Ki67

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Recombinant fusion protein of human Ki67.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Andere Bezeichnung: MKI67

Hintergrund: Antigen KI-67

Gen-ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process