Integrin beta 7 Antikörper

Produktnummer ABIN7840105

Der Ratte Monoklonal anti-Integrin beta 7 Antikörper wird verwendet zum Nachweis von Integrin beta 7 in Proben von Human und Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für Integrin beta 7 Antikörper (ABIN7840105)

Target: Integrin beta 7 (ITGB7)

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 7 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: FIB21

Produktdetails anti-Integrin beta 7 Antikörper

Verwendungszweck: Rat monoclonal antibody to Integrin beta 7

Spezifität: Recognizes human/mouse Integrin beta 7

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: TK1 cells

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu Integrin beta 7

Target: Integrin beta 7 (ITGB7)

Andere Bezeichnung: ITGB7

Hintergrund: Integrin beta-7, Gut homing receptor beta subunit

Gen-ID: 3695, 16421

UniProt: P26010

Pathways: Integrin Complex