Interleukin 17a Antikörper (PE)

Produktnummer ABIN7840015

Der Maus Monoklonal anti-Interleukin 17a Antikörper wird verwendet zum Nachweis von Interleukin 17a in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für Interleukin 17a Antikörper (PE) (ABIN7840015)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interleukin 17a Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: BL23

Produktdetails anti-Interleukin 17a Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to IL-17A

Spezifität: Recognizes human IL-17A

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Recombinant full length human IL-17A

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: IL17A

Hintergrund: CTLA8, IL17, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

Gen-ID: 3605

UniProt: Q16552