HLA-B27 Antikörper (PE)

Produktnummer ABIN7839659

Der PE-konjugierte Maus Monoklonal anti-HLA-B27 Antikörper (Klon HLA-B27) (ABIN7839659) detektiert spezifisch HLA-B27 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HLA-B27 Antikörper (PE) (ABIN7839659)

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-B27 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HLA-B27

Produktdetails anti-HLA-B27 Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to HLA-B27

Spezifität: Recognizes human HLA-B27

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Immune complex precipitated from HLA-B27 positive cell line (Bordin)

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu HLA-B27

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Andere Bezeichnung: HLA-B27

Hintergrund: HLA-B27 protein

UniProt: O19193

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response