HLA-B27 Antikörper

Produktnummer ABIN7839657

Dieser Maus Monoklonal Antikörper detektiert spezifisch HLA-B27 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für HLA-B27 Antikörper (ABIN7839657)

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-B27 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: ABCm3

Produktdetails anti-HLA-B27 Antikörper

Verwendungszweck: Mouse monoclonal antibody to HLA-B27

Spezifität: Recognizes human HLA-B27

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Immune complex precipitated from HLA-B27 positive cell line (Bordin)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu HLA-B27

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Andere Bezeichnung: HLA-B27

Hintergrund: HLA-B27 protein

UniProt: O19193

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response