CXCL9 Antikörper

Produktnummer ABIN7837366

Der Armenischer Hamster Monoklonal anti-CXCL9 Antikörper wird verwendet zum Nachweis von CXCL9 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für CXCL9 Antikörper (ABIN7837366)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CXCL9 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: MIG-2F5-5

Produktdetails anti-CXCL9 Antikörper

Verwendungszweck: Armenian Hamster monoclonal antibody to CXCL9

Spezifität: Recognizes mouse CXCL9

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse CXCL9 Recombinant Protein

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Andere Bezeichnung: CXCL9

Hintergrund: CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9

Gen-ID: 17329