CD99 Antikörper (FITC)
Kurzübersicht für CD99 Antikörper (FITC) (ABIN7836715)
Target
Alle CD99 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Mouse monoclonal antibody FITC labled to CD99
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Spezifität
- Recognizes human CD99
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Aufreinigung
- The antibody was purified by affinity chromatography.
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anti-CD99 (CD99) (AA 1-185) antibody
CD99 Reaktivität: Human, Maus FACS, IHC, WB, ELISA Wirt: Maus Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 Reaktivität: Human FACS, IHC, WB, ELISA, ICC Wirt: Maus Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 Reaktivität: Human IHC, WB, IF Wirt: Kaninchen Polyclonal unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 Reaktivität: Maus FACS, IHC, WB, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 Reaktivität: Ratte IHC, WB, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD99 (CD99) antibodyCD99 Reaktivität: Human FACS, MS Wirt: Maus Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 Reaktivität: Human FACS Wirt: Maus Monoclonal 3B2-TA8 PE
anti-CD99 (CD99) antibodyCD99 Reaktivität: Human IHC, WB, IHC (p) Wirt: Maus Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibodyKD Validated CD99 Reaktivität: Human FACS, WB Wirt: Kaninchen Monoclonal 23GB3395 unconjugated Recombinant Antibody
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Applikationshinweise
- Flow: 10 μL / assay
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Protokoll
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for one year. Do not freeze.
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- CD99
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Andere Bezeichnung
- CD99
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Hintergrund
- MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99
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Gen-ID
- 4267
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UniProt
- P14209
Target
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