CD90 Antikörper (FITC)
Kurzübersicht für CD90 Antikörper (FITC) (ABIN7836688)
Target
Alle CD90 (THY1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Rat monoclonal antibody FITC labled to CD90.2
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Spezifität
- Recognizes mouse CD902
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Aufreinigung
- The antibody was purified by affinity chromatography.
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Immunogen
- Mouse thymus or spleen
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anti-CD90 (THY1) (AA 17-132) antibody
THY1 Reaktivität: Human WB, IHC, ICC, ELISA Wirt: Maus Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD90 (THY1) (AA 31-120) antibodyTHY1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schaf FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB03970 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3F9 unconjugated
anti-CD90 (THY1) antibodyTHY1 Reaktivität: Human, Affe, Pavian FACS, IHC, IP, IHC (fro), IHC (p) Wirt: Maus Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 Reaktivität: Human, Schwein, Pferd, Non-Human Primate FACS Wirt: Maus Monoclonal 5E10 FITC
anti-CD90 (THY1) antibodyTHY1 Reaktivität: Maus FACS, CyTox Wirt: Maus Monoclonal F7D5 unconjugated
anti-CD90 (THY1) antibodyGMP Grade THY1 Reaktivität: Human FACS Wirt: Maus Monoclonal F15-42-1 unconjugated
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Applikationshinweise
- Flow: 10 μL / assay
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Protokoll
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for one year. Do not freeze.
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- CD90 (THY1)
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Andere Bezeichnung
- THY1
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Hintergrund
- Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90
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Gen-ID
- 21838
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Pathways
- Cell-Cell Junction Organization
Target
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