CD9 Antikörper (Biotin)

Produktnummer ABIN7836672

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD9 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD9 Antikörper (Biotin) (ABIN7836672)

Target: CD9

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD9 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HI9a

Produktdetails anti-CD9 Antikörper (Biotin)

Verwendungszweck: Mouse monoclonal antibody Biotin labled to CD9

Spezifität: Recognizes human CD9

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD9.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD9

Target: CD9

Andere Bezeichnung: CD9

Hintergrund: MIC3, TSPAN29, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD antigen CD9

Gen-ID: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization