CD69 Antikörper (PE-Cy7)

Produktnummer ABIN7836555

Dieser Armenischer Hamster Monoklonal Antikörper detektiert spezifisch CD69 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für CD69 Antikörper (PE-Cy7) (ABIN7836555)

Target: CD69

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD69 Antikörper ist konjugiert mit PE-Cy7

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: H1-2F3

Produktdetails anti-CD69 Antikörper (PE-Cy7)

Verwendungszweck: Armenian Hamster monoclonal antibody PE/Cy7 labled to CD69

Spezifität: Recognizes mouse CD69

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse dendritic epidermal T cell line Y245

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD69

Target: CD69

Andere Bezeichnung: CD69

Hintergrund: CLEC2C, Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69

Gen-ID: 12515