CEACAM5 Antikörper

Produktnummer ABIN7836538

Der Maus Monoklonal anti-CEACAM5 Antikörper wird verwendet zum Nachweis von CEACAM5 in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für CEACAM5 Antikörper (ABIN7836538)

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CEACAM5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: CB30

Produktdetails anti-CEACAM5 Antikörper

Verwendungszweck: Mouse monoclonal antibody to CD66e

Spezifität: Recognizes human CD66e

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Andere Bezeichnung: CEACAM5

Hintergrund: CEA, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e

Gen-ID: 1048

UniProt: P06731