L-Selectin Antikörper (APC)

Produktnummer ABIN7836511

Der Ratte Monoklonal anti-L-Selectin Antikörper wird verwendet zum Nachweis von L-Selectin in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für L-Selectin Antikörper (APC) (ABIN7836511)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: MEL-14

Produktdetails anti-L-Selectin Antikörper (APC)

Verwendungszweck: Rat monoclonal antibody APC labled to CD62L

Spezifität: Recognizes mouse CD62L

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: C3H/eb mouse B lymphoma 38C-13

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: SELL

Hintergrund: LNHR, LYAM1, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, TQ1, gp90-MEL, CD62L

Gen-ID: 20343