Selectin E/CD62e Antikörper (Alexa Fluor 700)

Produktnummer ABIN7836502

Der Maus Monoklonal anti-Selectin E/CD62e Antikörper wird verwendet zum Nachweis von Selectin E/CD62e in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für Selectin E/CD62e Antikörper (Alexa Fluor 700) (ABIN7836502)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Selectin E/CD62e Antikörper ist konjugiert mit Alexa Fluor 700

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 1-2B6

Produktdetails anti-Selectin E/CD62e Antikörper (Alexa Fluor 700)

Verwendungszweck: Mouse monoclonal antibody AF700 labled to CD62E/CD62P

Spezifität: Recognizes human CD62E/CD62P

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: TNF stimulated human endothelial cells

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: SELE

Hintergrund: ELAM1, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E, GMRP, GRMP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P

Gen-ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling