ITGA6 Antikörper (PE)

Produktnummer ABIN7836427

Der Ratte Monoklonal anti-ITGA6 Antikörper wird verwendet zum Nachweis von ITGA6 in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für ITGA6 Antikörper (PE) (ABIN7836427)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA6 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 4F10

Produktdetails anti-ITGA6 Antikörper (PE)

Verwendungszweck: Rat monoclonal antibody PE labled to CD49f

Spezifität: Recognizes human CD49f

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD49f.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Andere Bezeichnung: ITGA6

Hintergrund: Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD antigen CD49f)

Gen-ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex