GP1BB Antikörper (PE-Cy5)

Produktnummer ABIN7836304

Der Maus Monoklonal anti-GP1BB Antikörper wird verwendet zum Nachweis von GP1BB in Proben von Human. Er wurde validiert für IF und FACS.

Kurzübersicht für GP1BB Antikörper (PE-Cy5) (ABIN7836304)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GP1BB Antikörper ist konjugiert mit PE-Cy5

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: MM2-174

Produktdetails anti-GP1BB Antikörper (PE-Cy5)

Verwendungszweck: Mouse monoclonal antibody PE/Cy5 labled to CD42b

Spezifität: Recognizes human CD42b

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Andere Bezeichnung: GP1BB

Hintergrund: Platelet glycoprotein Ib beta chain, GP-Ib beta, GPIb-beta, GPIbB, Antigen CD42b-beta, CD42c

Gen-ID: 2812

UniProt: P13224