GP1BB Antikörper

Produktnummer ABIN7836300

Dieser Maus Monoklonal Antikörper detektiert spezifisch GP1BB in IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GP1BB Antikörper (ABIN7836300)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GP1BB Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: HIP1

Produktdetails anti-GP1BB Antikörper

Verwendungszweck: Mouse monoclonal antibody to CD42b

Spezifität: Recognizes human CD42b

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD42b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Andere Bezeichnung: GP1BB

Hintergrund: Platelet glycoprotein Ib beta chain, GP-Ib beta, GPIb-beta, GPIbB, Antigen CD42b-beta, CD42c

Gen-ID: 2812

UniProt: P13224