CD4 Antikörper (Pacific Blue)

Produktnummer ABIN7836272

Der Pacific Blue-konjugierte Ratte Monoklonal anti-CD4 Antikörper (Klon GK1-5) (ABIN7836272) detektiert spezifisch CD4 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für CD4 Antikörper (Pacific Blue) (ABIN7836272)

Target: CD4

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit Pacific Blue

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: GK1-5

Produktdetails anti-CD4 Antikörper (Pacific Blue)

Verwendungszweck: Rat monoclonal antibody Pacific Blue labled to CD4

Spezifität: Recognizes mouse CD4

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified mouse CD4.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD antigen CD4

Gen-ID: 12504

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events